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Nucleic Acids Research, 1977, Vol. 4, No. 9 2997-3006
© 1977

Articles

Antibodies specific to a deoxyribodinucleotide sequence*

Saleem A. Khan+, M. Zafri Humayun+ and T. M. Jacob{dagger}

Department of Biochemistry, Indian Institute of Science, Bangalore-560012, India

Received May 6, 1977.

Antibodies were raised in rabbits against the bovine serum albumin conjugate of dpApT. Analysis by double diffusion in agar gel and quantitative precipitation test showed the presence of antibodies specific to the hapten in the antisera. Quantitative data on the specificity of the antibodies were obtained by studying the inhibition of the binding of 3H-dpApT to the anti-sera by various nonradioactive mono- and oligonucleotides, using a nitrocellulose membrane binding assay. The antibodies were found to be highly specific for the dinucleotide sequence dpApT. The antibodies were able to bind to synthetic oligonucleotides containing the sequence dpApT and to denatured calf thymus DNA.

*This is no. 5 of the series entitled " Immunological studies on nucleic acids and their components ".

+Present address: Department of Biochemistry New York University School of Medicine 550 First Avenue, New York, N.Y. 10016, U.S.A.

{dagger}Principal investigator


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