Skip Navigation

This Article
Right arrow Print PDF (1486K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (48)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Zan-Kowalczewska, M.
Right arrow Articles by Shatkin, A. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zan-Kowalczewska, M.
Right arrow Articles by Shatkin, A. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1977, Vol. 4, No. 9 3065-3082
© 1977

Articles

Removal of 5'-terminal m7G from eukaryotic mRNAs by potato nucleotide pyrophosphatase and its effect on translation

Malgorzata Zan-Kowalczewska+, Maria Bretner+, Halina Sierakowska+, Elzbieta Szczesna{dagger}, Witold Filipowicz{dagger} and A. J. Shatkin*

+Department of Molecular Biology 02-532 Warsaw, Poland {dagger}Department of Protein Biosynthesis, Institute of Biochemistry and Biophysics, The Polish Academy of Sciences 02-532 Warsaw, Poland *Roche Institute of Molecular Biology Nutley, NJ 07110, USA

Received June 8, 1977. The procedure for isolation of nucleotide pyrophosphatase (E.C. 3.6.1.9 [EC] .) from potato has been modified to yield an endonuclease-free preparation purified 2300–fold. The enzyme was used for specific cleavage of pyrophosphate linkages in the 5'-terminal cap (m7GpppN) of several eukaryotic messenger RNAs. Enzymatic removal of 5'-terminal pm7G from reovirus, rabbit globin and Artemia salina mRNAs resulted in an almost complete loss (>80%) of their template activities in a cell-free protein synthesizing system from wheat germ. Incubation with nucleotide pyrophosphatase did not decrease the translation of phage f2 RNA in an Escherichia coli cell-free system.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Virol.Home page
Z. W. Whitlow, J. H. Connor, and D. S. Lyles
Preferential Translation of Vesicular Stomatitis Virus mRNAs Is Conferred by Transcription from the Viral Genome
J. Virol., December 1, 2006; 80(23): 11733 - 11742.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.