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Nucleic Acids Research, 1977, Vol. 4, No. 9 3239-3258
© 1977

Articles

Primary structure of baker's yeast tRNA2bVal

Valentine G. Gorbulev, Vladimir D. Axel'rod and Alexander A. Bayev

Institute of Molecular Biology, Academy of Sciences of the USSR Vavilov Str., 32, Moscow, B-312, USSR

Received July 25, 1977. The minor form of valine tRNA from baker's yeast – tRNA2bVal – purified by column chromatography was completely digested with guanylo–RNase and pancreatic RNase. The products of these digestions were separated by a combination of thin-layer chromatography on cellulose and high voltage electrophoresis on DEAE–paper and then identified. The halves of tRNA2bVal were prepared by partial digestion with pancreatic RNase and their complete guanylo–RNase and pancreatic RNase, digests were analysed. Basing on the obtained data the primary structure of baker's yeast tRNA2bVal was reconstructed.


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