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Nucleic Acids Research, 1978, Vol. 5, No. 1 1-11
© 1978

Articles

Studies of the transcription of viral genome in adenovirus 5 transformed cells

E.I. Frolova, E.S. Zalmanzon, E.M. Lukanidin and G.P. Georgiev

Institute of Molecular Biology, Academy of Sciences of the USSR Moscow, USSR

Received August 8, 1977. Transcription of the human adenovirus 5 genome in transformed rat embryo cells (DFK3) was investigated using two different approaches. Preferential digestion of transcribed viral sequences by DNase I was analysed using kinetics of renaturation of 32P-labeled Ad5 HpaI restriction fragments in the presence of material which was stable after nuclease treatment. The second approach was the hybridization of 32P-labeled nuclear RNA from transformed cells with Ad5 restriction fragments which were attached to a nitrocellulose filter. These two methods gave similar results. It was found that not all integrated regions of the Ad5 genome are active in transformed cells.

2,5 copies of the HpaI-E fragment of Ad5 DNA were found in transformed DFK3 cell line. Nuclear RNA from these cells hybridized to HpaI-E fragment of Ad5 DNA, but only about half of sequences of the integrated HpaI-E fragment was sensitive to DNase I digestion.


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