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Nucleic Acids Research, 1978, Vol. 5, No. 1 195-204
© 1978

Articles

Two initiation sites for adenovirus 5.5S RNA

Björn Vennström, Ulf Pettersson and Lennart Philipson

Department of Microbiology, Biomedical Center, Uppsala University Uppsala, Sweden

Received October 31, 1977. Adenovirus specific 5.5S RNA is heterogeneous at its 5' terminus. Complete pancreatic RNase digests of the RNA reveal a 5' terminal oligonucleotide (pp)pApGpCp in addition to the major 5' terminal (pp)pGpGpGpCp (1). Both 5' termini are detected early as well as late after adenovirus infection. In isolated nuclei, {alpha}-amanitin inhibits all 5.5S RNA transcription at a concentration of 200 µg/ml, indicating that both initiation sites are recognized by RNA polyrnerase III.


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