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Nucleic Acids Research, 1978, Vol. 5, No. 1 297-303
© 1978

Articles

Affinity adsorbents consisting of nucleic acids immobilized via bisoxirane activated polysaccarides

Hans Potuzak and Peter D.G. Dean

Department of Biochemistry, University of Liverpool Liverpool L69 3BX, UK

Correspondence and requests for reprints to: Hans Potuzak Present address: Institute of Molecular Biology, Wasagasse 9, A-1090 Vienna, AUSTRIA

Received December 2, 1977. An easy and efficient procedure for the immobilization of polynucleotide liganda to bisoxirane activated insoluble polysaccharides has been elaborated and is described in this paper. The resulting materials have been applied to the chromatography of DNA polymarase I, and RNA polymerase from E.coli. Because of their extraordinary stability to temperature, formamide, and alka-line conditions they seem to be particularly useful adsorbents for nucleic acid hybridization.


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