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Nucleic Acids Research, 1978, Vol. 5, No. 10 3457-3468
© 1978

Articles

A specific endonuclease from Bacillus caldolyticus

Alistair H.A. Bingham, Tony Atkinson, Daniela Sciaky* and Richard J. Roberts*

Microbiological Research Establishment Porton, Salisbury, Wiltshire, UK *Cold Spring Harbor Laboratory Cold Spring Harbor, NY 11724, USA

Received August 14, 1978. The purification and characterization of a new restriction endonuclease, BclI from the extreme thermophile Bacillus caldolyticus is reported. This enzyme recognizes the sequence

5'-T{downarrow}-G-A-T-C-A-3'

3'-A-C-T-A-G{uparrow}-T-5'

and cleaves at the positions indicated by the arrows.


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