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Nucleic Acids Research, 1978, Vol. 5, No. 10 3549-3564
© 1978

Articles

Specific termination of RNA polymerase synthesis as a method of RNA and DNA sequencing

Vladimir Axelrod, Robert M. Vartikyan, Vissarion A. Aivazashvili and Robert Sh. Beabealashvili

Institute of Molecular Biology, USSR Academy of Sciences Vavilova str., Moscow B-334, USSR

Received July 28, 1978. Termination of RNA synthesis with 3'-O-Methylnucleoside 5'-triphosphates have been studied using E.coli RNA polymerase holoenzyme and poly [d(A-T)] as well as unfractionated T7 D {Delta} III DNA as templates. It was shown that the termination can be used for DNA sequencing. A sequence of a part of RNA synthesized from AI promoter of the DNA have been determined. Syntheses of four 3'-O-Methylnucleoside 5'-triphosphates are described.


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