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Nucleic Acids Research, 1978, Vol. 5, No. 10 3831-3842
© 1978

Articles

Purification of potential 3' processing nucleases using synthetic tRNA precursors*

Ranajit K. Ghosh+ and Murray P. Deutscher

Department of Biochemistry, University of Connecticut Health Center Farmington, CT 06032, USA +Indian Institute of Experimental Medicine Jadavpur, Calcutta, India.

Received July 27, 1978.

The synthetic tRNA precursors, tRNA-C-[14C]U and tRNA-C-C-A-[14C]C-C, as well as poly(A) and diesterase-treated tRNA, have been used to identify and purify potential 3'processing nucleases. Four activities have been separated by this analysis; and three of them have been characterized. Two of the enzymes, which are well-separated on hydroxylapatite columns, act on poly(A), require K+ and Mg2+ for activity, and have molecular weights of about 90,000. These activities have properties previously ascribed to RNase II. The third enzyme does not act on poly(A), requires Mg2+ for activity, and has a molecular weight of about 60,000, It is identical to RNase D, previously characterized as an exonuclease acting on tRNAs with altered structure. Each of the enzymes can remove nucleotides from the tRNA precursor containing extra nucleotides beyond the 3'terminus, whereas they are relatively inactive with intact tRNA or tRNA-C-U. The greatest specificity was displayed by RNase D. The possibility that RNase D is a 3'processing nuclease is discussed.

*This paper is number 25 in the series, "Reactions at the 3'Terminus of tRNA". The previous paper in the series is reference 10.


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