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Nucleic Acids Research, 1978, Vol. 5, No. 10 3945-3958
© 1978

Articles

DNA polymerase {alpha} and ß in the California urchin

Francis M. Racine and Paul W. Morris

Department of Biological Chemistry, University of Illinois Medical Center Chicago, IL 60612, USA

Received June 19, 1978. DNA polymerase {alpha} and ß were identified in the urchin, Strongylocentrotus purpuratus. The DNA polymerase ß sedimented at 3.4 S, constituted 5% of total DNA polymerase activity, and was resistant to N-ethylmaleimide and high ionic strength. The polymerase {alpha} sedimented at 6-8 S, was inhibited by N-ethylmaleimide or 0.1 M (NH4)2SO4 and was dependent upon glycerol for preservation of activity. Both the polymerases {alpha} and ß were nuclear associated in embryos. The DNA polymerase {alpha} was markedly heterogeneous on DEAE-Sephadex ion exchange and showed three modal polymerase species. These polymerase a species were indistinguishable by template activity assays but the DNA polymerase associated ribonucleotidyl transferase (Biochemistry 75:3106-3113, 1976) was found predominantly with only one of the DNA polymerase {alpha} species.


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