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Nucleic Acids Research, 1978, Vol. 5, No. 11 4055-4064
© 1978

Articles

Recognition sequence of restriction endonuclease KpnI from Klebsiella pneumoniae

Joanne Tomassini, Ranajit Roychoudhury, Ray Wu* and Richard J. Roberts{dagger}

Section of Biochemistry, Molecular and Cell Biology, Cornell University Ithaca, NY 14853 {dagger}Cold Spring Harbor Laboratory P.O. Box 100, Cold Spring Harbor, NY 11724, USA

* Individual to whom correspondence should be sent.

Received August 24, 1978. We have determined the recognition sequence of the restriction endonuclease KpnI, previously isolated from Kiebsiella pneumoniae1. The enzyme cleaves the twofold rotationally symmetric sequencev Formula at the positions indicated by the arrows, producing 3' protruding cohesive ends, four nucleotides in length. The specific cleavage site was unambiguously deduced using both 3' and 5' end analyses of KpnI generated restriction fragments of simian-virus 40 (SV4O) DNA (1 site), adenovirus-2 (Ad-2) DNA (8 sites), and a plasmid (pCRI) DNA (2 sites).


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