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Nucleic Acids Research, 1978, Vol. 5, No. 11 4077-4086
© 1978

Articles

The 1360 bp long basic repeat unit of calf satellite I DNA contains GC rich nucleus of about 140 bp

Jacek Skowronski, Krystyna Furtak, Janusz Klysik, Henryk Panusz and Andrzej Plucienniczak

Department of Biochemistry, Institute of Physiology and Biochemistry, Medical Academy 90–131 Lódz Lindleya 6, Poland

Received August 8, 1978. Fine melting profiles of calf satellite I DNA and its fragments obtained after digestion with endoR.EcoRI and endoR. AluI nucleases were investigated. It is shown that the 1360 bp basic repeat unit of calf satellite I DNA contains an about 140 bp long GC rich nucleus. It is localized on the 600 bp restriction fragment obtained after digestion of 1360 bp fragment with endoR,AluI nuclease.

The main part of satellite I DNA melts as loops between such GC rich nuclei which strongly influence the melting properties of this satellite. There exist significant differences between the thermal stabilities of fragments containing many nuclei, one nucleus and those in which such nucleus is absent.


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