Skip Navigation

This Article
Right arrow Print PDF (1258K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (50)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Gingeras, T.R.
Right arrow Articles by Roberts, R.J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Gingeras, T.R.
Right arrow Articles by Roberts, R.J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1978, Vol. 5, No. 11 4105-4128
© 1978

Articles

A computer assisted method for the determination of restriction enzyme recognition sites

T.R. Gingeras, J.P. Milazzo* and R.J. Roberts

Cold Spring Harbor Laboratory Cold Spring Habor, NY 11724 *Computing Center, State University of New York Stony Brook, NY 11794, USA

Received August 7, 1978. A computer program has been developed which aids in the determination of restriction enzyme recognition sequences. This is achieved by cleaving DNAs of known sequence with a restriction endonuclease and comparing the fragmentation pattern with a computer-generated set of patterns. The feasibility of this approach has been tested using fragmentation patterns of øX174 DNA produced by enzymes of both known and unknown specificity. Recognition sequences are predicted for two restriction endonucleases (BbvI and SfaNI) using this method. In addition, recognition sequences are predicted for two other new enzymes (PvuI and MstI) using another computer-assisted method.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Nucleic Acids ResHome page
J. Ryu and E. Rowsell
Quick identification of Type I restriction enzyme isoschizomers using newly developed pTypeI and reference plasmids
Nucleic Acids Res., August 1, 2008; 36(13): e81 - e81.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
R. J. Roberts, T. Vincze, J. Posfai, and D. Macelis
REBASE--restriction enzymes and DNA methyltransferases
Nucleic Acids Res., January 1, 2005; 33(suppl_1): D230 - D232.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. K. A. Kasarjian, M. Hidaka, T. Horiuchi, M. Iida, and J. Ryu
The recognition and modification sites for the bacterial type I restriction systems KpnAI, StySEAI, StySENI and StySGI
Nucleic Acids Res., June 15, 2004; 32(10): e82 - e82.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
J. K. A. Kasarjian, M. Iida, and J. Ryu
New restriction enzymes discovered from Escherichia coli clinical strains using a plasmid transformation method
Nucleic Acids Res., March 1, 2003; 31(5): e22 - e22.
[Abstract] [Full Text] [PDF]

Home page
 J. Am. Med. Inform. Assoc.Home page
T. C. Rindfleisch and D. L. Brutlag
Directions for Clinical Research and Genomic Research into the Next Decade: Implications for Informatics
J. Am. Med. Inform. Assoc., September 1, 1998; 5(5): 404 - 411.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
T. Gingeras and R. Roberts
Steps toward computer analysis of nucleotide sequences
Science, September 19, 1980; 209(4463): 1322 - 1328.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
Q. Xu, S. Stickel, R. J. Roberts, M. J. Blaser, and R. D. Morgan
Purification of the Novel Endonuclease, Hpy188I, and Cloning of Its Restriction-Modification Genes Reveal Evidence of Its Horizontal Transfer to the Helicobacter pylori Genome
J. Biol. Chem., May 26, 2000; 275(22): 17086 - 17093.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.