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Nucleic Acids Research, 1978, Vol. 5, No. 11 4177-4194
© 1978

Articles

RNA synthesis in myeloma cells synchronized by isoleucine starvation

William F. Marzluff, Jr.1, Chi-Jiunn Pan and Dave L. Cooper

Department of Chemistry, Florida State University Tallahassee, FL 32306, USA

Received July 21, 1078.

Myeloma cells have been synchronized by isoleucine starvation. Changes in RNA synthetic rates as a result of starvation have been studied. The ability of isolated nuclei to synthesize RNA declines on starvation and increases subsequently on refeeding isoleucine. There is a coordinate drop in synthetic rate for all three polymerases both in vivo and in vitro. The chain elongation rate in vitro is the same in starved and normal cells, so the difference is in the number of active polymerases in vitro However, the nuclei do not exactly parallel the state of the cell from which they were isolated, but the in vitro RNA synthesis increases more slowly than the in vivo RNA synthesis. There is no change in relative amounts of synthesis by the different RNA polymerases. The in vitro RNA product is similar in starved and growing cells.

1Marzluff, Pan and Cooper, manuscript in preparation


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