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Nucleic Acids Research, 1978, Vol. 5, No. 12 4547-4562
© 1978

Articles

The ovalbumin split gene: molecular cloning of Eco RI fragments "c" and "d"

J.P. LePennec*, P. Baldacci**, F. Perrin*, B. Cami**, P. Gerlinger*, A. Krust*, P. Kourilsky** and P. Chambon*

*Lab. Génétique Moléculaire des Eucaryotes du CNRS, U. 44 de I'INSERM, Institut de Chimie Biologique, Faculté de Medecine 11, Rue Humann, Strasbourg 67085 **Groupe de Biologie Moléculaire du Gène ERA-CNRS 201, Institut Pasteur, 25, Rue du Dr. Roux, Paris, France

Received October 16, 1978. The Eco RI fragments "c" and "d" of the ovalbumin gene (1, 2) have been isolated by molecular cloning. Restriction enzyme mapping and electron microscopy have confirmed that the two fragments contain the same ovalbumin mRNA coding sequences. These sequences are split into two regions which have been mapped in fragments "c" and "d". There is no evidence that the ovalbumin mRNA sequences contained in these fragments could be further interrupted. Our results confirm that the presence of Eco RI fragment "d" in some chickens is due to the existence of an allelic variant of the ovalbumin gene which contains an additional Eco RI site within the region corresponding to Eco RI fragment "c". This additional Eco RI site appears to be the main difference between the two alleles. Finally, our results provide a direct demonstration that most of the ovalbumin mRNA sequences are encoded for by Eco RI fragments "a", "b" and "c".


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