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Nucleic Acids Research, 1978, Vol. 5, No. 12 4563-4578
© 1978

Articles

A conserved and unique (AT)-rich segment in yeast mitochondrial DNA

J.L. Bos, C.F. Van Kreiji*, F.H. Ploegaert, J.N.M. Mol** and P. Borst

Section for Medical Enzymology and Molecular Biology, Laboratory of Biochemistry, University of Amsterdam, Jan Swammerdam Institute P.O. Box 60.000, 1005 GA Amsterdam, Netherlands

Received October 16, 1978.

The mtDNA of the cytoplasmic petite mutant of yeast RD1A consists mainly of a perfect head-to-tail repetition of a known sequence of 66 consecutive AT and 2 GC base pairs. We have hybridized complementary RNA made on RD1A mtDNA with the mtDNAs of four different wild-type Saccharomyces strains that differ markedly in restriction fragmentation pattern. The tm'S of the four heteroduplexes are identical to the tm of the homoduplex of RD1A mtDNA with complementary RNA of one repeat length. With all four wild-type mtDNAs this complementary RNA hybridizes mainly to a single restriction fragment of about 300 base pairs. This shows the conservation and individuality of at least one (AT)-rich segment in yeast mtDNA. The 300 base pair fragment has been mapped in the vicinity of the oxi-2 locus. The possible role of the (AT)-rich segment in the processing of the primary transcript of this region is discussed.

*Present address: Rijks Instituut voor Drinkwatervoorziening, Laboratorium voor Microbiologie, P.O. Box 150, 2260 AD Leidschendam, Netherlands.

**Present address: Erasmus Universiteit Rotterdam, Laboratorium voor Pathologische Anatomie II, P.O. Box 1738, 3000 DR Rotterdam, Netherlands.


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