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Nucleic Acids Research, 1978, Vol. 5, No. 12 4805-4818
© 1978

Articles

An octamer of histones H3 and 114 forms a compact complex with DNA of nucleosome size

Richard H. Simon, R. Daniel Camerini-Otero* and Gary Felsenfeld

Laboratory of Molecular Biology Bethesda, MD 20014, USA *Section on Human Biochemical Genetics, National Institute of Arthritis, Metabolism and Digestive Diseases, National Institutes of Health Bethesda, MD 20014, USA

Received October 3, 1978. Equimolar mixtures of histones H3 and H4 have been reconstituted onto DNA of nucleosome core size. Two distinct complexes are formed in a relative abundance that depends on the starting ratio of H3 $ H4 to DNA. One of these complexes contains two H3–H4 dimers for each DNA molecule, and has a sedimentation coefficient of 7.5S. The other complex contains an octamer consisting of four H3–H4 dimers, and has a sedimentation coefficient of 10.4S. On the basis of these measurements, we conclude that the octamer complex (but not the tetramer complex) is a fully folded, compact structure resembling the nucleosome.


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