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Nucleic Acids Research, 1978, Vol. 5, No. 2 317-330
© 1978

Articles

Restriction endonuclease cleavage map of mitochondrial DNA from Aspergillus nidulans

Piotr P. Stepien, Ulrich Bernard1, Howard J. Cooke2 and Hans Küntzel

Max-Planck-Institut für experimentel Medizin, Abteilung Chemie, D-3400 Göttingen Hermann-Rein-Strasse 3, GFR

Received October 21, 1977.

Mitochondrial DNA of the ascomycete fungus Aspergillus nidulans, a circular molecule of 31 500 base pairs, is cleaved by restriction endonucleases Eco R I, Hind II, Hind III and Bgl II into 3,7,9 and 5 fragments, respectively. The relative positions of the cleavage sites could be mapped by analysis of fragments obtained by double enzyme digestions of whole DNA and by complete and partial redigestion of isolated restriction fragments.

1 Present address: Department of Biology B-022, University of California San Diego, La Jolla, California 92093, USA

2 Permanent address: Medical Research Council, Mammalian Genome Unit, Department of Zoology, University of Edinburgh, West Mains Road, Edinburgh, U.K.


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