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Nucleic Acids Research, 1978, Vol. 5, No. 2 425-434
© 1978

Articles

Isolation from rat liver and sequence of a RNA fragment containing 32 nucteotides from position 5 to 36 from the 37' end of ribosomal 18S RNA

Heidemarie Alberty, Manfred Raba and Hans J. Gross

Max-Planck-Institut für Biochemie D-8033 Martinsried bei München, GFR

Received December 5, 1977. Crude tRNA isolated from rat liver by the method of Rogg et al. (Biochem. Biophys. Acta 195, 13–15 1969) contains N6-dimethyl-a denosine (Formula ) and was therefore fractionated in order to identify the Formula -containing RNAs. A unique species of RNA was purified which contained all the Formula present in the crude tRNA. Sequence analysis by postlabeling with {gamma}-32P-ATP and polynucleotide kinase revealed that this RNA represents the 32 nucleotides


Formula

from position 5 to 36 of the 3' terminus of ribosonal 18S RNA. The 36 nucleotide long sequence from the 3' end of rat liver 18S rRNA exhibits extensive homology with the corresponding sequence of E. coli 16S rRNA and with the 21 nucleotide long 3' terminal equence so far known from Saccharomyces carlsbergensis 17S rRNA. A heterogeneity in this sequence provides the first evidence on the molecular level for the existence of (at least) two sets of redundant ribosomal 18S RNA genes in the rat.


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