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Nucleic Acids Research, 1978, Vol. 5, No. 2 583-590
© 1978

Articles

A rapid method for the isolation of circular DNA using an aqueous two-phase partition system

R. Ohlsson+, C. C. Hentschel* and J. G. Williams*

+National Institute for Medical Research The Ridgeway, Mill Hill, London, NW7 1AA *Imperial Cancer Research Fund, Burtonhole Lane Mill Hill, London, NW7 1AD, UK

Received December 23, 1977. A method of isolating circular plasmid DNA from cleared lysates of E.coli is described. Purification is achieved by virtue of the rapid re-annealing kinetics of supercoiled DNA. After a brief denaturation step, double stranded plasmid DNA is separated from denatured chromosomal DNA and RNA in a two-phase partition system using dextran and polyethylene glycol. The method is much more rapid than the conventional dyecentrifugation technique and plasmid DNA of comparalle purity and yield is obtained.


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