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Nucleic Acids Research, 1978, Vol. 5, No. 4 1153-1163
© 1978

Articles

A second site-specific restriction endonuclease from Staphylococcus aureus

J. S. Sussenbach, P. H. Steenbergh, J. A. Rostx, W. J. van Leeuwenx and J. D. A. van Embdenx

Laboratory for Physiological Chemistry, State University of Utrecht Utrecht xNational Institute of Public Health P.O. Box 1, Bilthoven, Netherlands

Received February 6, 1978. A site-specific restriction endonuclease has been isolated from Staphylococcus aureus PS 96. This enzyme, Sau96 I, recognizes the DNA sequence 5'--G'-G-N-C-C--3'3'p--C-CN'-G-G--5' and cleaves as indicated by the arrows. The enzyme cleaves adenovirus type 5 and {lambda} DNA many times, SV40 DNA 10 times and ØX174 RF DNA 2 times. Evidence is presented that the enzyme is involved in biological restriction-modification.


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