Skip Navigation

This Article
Right arrow Print PDF (631K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Quinlan, T. J.
Right arrow Articles by Getz, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Quinlan, T. J.
Right arrow Articles by Getz, M. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1978, Vol. 5, No. 5 1611-1625
© 1978

Articles

The concept of mRNA abundance classes: a critical reevaluation

Thomas J. Quinlan*, George W. Beeler, Jr.{dagger}, Ronald F. Cox+, Paula K. Elder*, Harold L. Moses*,+ and Michael J. Getz*

*Departments of Pathology and Anatomy, Mayo Clinic and Mayo Foundation Rochester, MN 55901, USA {dagger}Departments of Physiology and Biophysics, Mayo Clinic and Mayo Foundation Rochester, MN 55901, USA +Department of Molecular Medicine, Mayo Clinic and Mayo Foundation Rochester, MN 55901, USA

Received February 20, 1978. Derivative plots have been constructed for hybridization reactions between polysomal poly(A)-containing RNA and oligo(dT)-primed cDNA. In one method the derivative was calculated directly from the data, and in the other, from a non-linear least squares fit using 9–10 ideal components. In some cases these methods yield very similar results and strongly suggest that the hybridization data support discrete components. Reactions with two and four major components indicate that the often-reported three abundance class model is only one of several possibilities for eukaryotic cells. In other Situations neither method strongly suggests the presence of discrete components (in one case even after enrichment of the cDNA population by kinetic fractionation), implying that the components are closely spaced or that the entire mRNA population of those cells may not exist as discrete abundance classes. The universal occurrence of discrete abundance classes should be critically reexamined.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Genome ResHome page
J. Stollberg, J. Urschitz, Z. Urban, and C. D. Boyd
A Quantitative Evaluation of SAGE
Genome Res., August 1, 2000; 10(8): 1241 - 1248.
[Abstract] [Full Text]

Home page
 ScienceHome page
N Chaudhari and W. Hahn
Genetic expression in the developing brain
Science, May 27, 1983; 220(4600): 924 - 928.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.