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Nucleic Acids Research, 1978, Vol. 5, No. 5 1701-1713
© 1978

Articles

Replacement of wybutine by hydrazines and its effect on the active conformation of yeast tRNAPhe

Hans-George Schleich, Wolfgang Wintermeyer and Hans G. Zachau

Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie der Universität München Goethestrasse 33, D-8000 München 2, GFR

Received March 14, 1978. The highly modified base wybutine (YWye)2 next to the anticodon of yeast tRNAPhe has been replaced by different hydrazine derivatives. The effect of the replacement on the activity of the tRNA has been studied in the heterologous aminoacylation with synthetase from E. coli and in the poly (U) directed binding to ribosomes from both yeast and E. coli. It was found that starting from tRNAPhe-YWye the activity increased with increasing size, aromaticity, and stacking tendency of the substituent replacing YWye. It is concluded that YWye by the size of its aromatic system and by its stacking properties is particularly well suited for stabilizing the native conformation of tRNAPhe.


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