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Nucleic Acids Research, 1978, Vol. 5, No. 6 1767-1777
© 1978

Articles

Construction of recombinant plasmid carrying the {lambda} DNA fragment responsible for prophage integration

N. Strizhov and L. Tikhomirova

Institute of Biochemistry and Physiology of Microorganisms, USSR Academy of Sciences Pushchino, 142292, USSR

Received April 4, 1978. The recombinant DNA molecules were constructed from plasmid RSF2124 and the EcoRI fragment of {lambda} DNA containing the genes responsible for prophage integration. The presence of these genes in recombinant plasmids was detected genetically. {lambda} int-gene was shown to be expressed in either orientation of insertion in the plasmid. We found that recombinant plasmid was able to integrate into chromosome of {lambda} lysogens. The integration of plasmid into host chromosome was demonstrated by contransduction of chromosome and plasmid markers using generalized transducer P1 and by specialized transduction with {lambda} phages.


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