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Nucleic Acids Research, 1978, Vol. 5, No. 6 1877-1887
© 1978

Articles

Polynucleotides. LII.1 Synthesis and properties of poly(2'-deoxy-2'-fluoroadenylic acid)

Mona Ikehara, Toshikazu Fukui and Nobuko Kakiuchi

Faculty of Pharmaceutical Sciences, Osaka University Suita, Osaka 565, Japan

Received April 11, 1978. 2'-Deoxy-2'-fluoroadenosine was chemically transformed to its 5'-diphosphate and polymerized with polynucleotide phosphorylase to give poly(2'-deoxy-2'-fluoroadenylic acid) (poly(Af)]. Polymerization proceeded smoothly as in the case of poly(A) and the yield of the polymerization was 55%. The UV absorption spectra of poly(Af) closely resembled those of poly(A) and the hypochromicity was 32% at pH 7.0. The CD profile at 25° and neutrality showed similar pattern to that of other poly(2'-deoxy-2'-halogenoadenylic acids) with somewhat larger [{theta}] values both in the positive and negative maxima. Acid titration of poly(Af) showed a transition point at pH 5.2 and the Tm of the acid form was 37° which was significantly lower than that of poly(A), but similar to that of poly(2'-azido-2'-deoxyade-nylic aicd). Poly(Af) formed 1:1 and 1:2 complexes with poly-(U) having Tm of 49° and 62° at 0.04M and 0.15M Na$ concentration, respectively. Poly(Af) also formed a 1:2 complex with poly(I) and its Tm was 36° at 0.05M Na$ concentration. These data showed that poly(Af) has rather similar properties to those of poly(A),but not to poly(dA).


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