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Nucleic Acids Research, 1978, Vol. 5, No. 7 2333-2344
© 1978

Articles

Characterization of the mRNA for herpes simplex virus thymidine kinase by cell-free synthesis of active enzyme

Kenneth Cremer, Monika Bodemer and William C. Summers

Departments of Therapeutic Radiology, Molecular Biophysics and Biochemistry, and Human Genetics, Yale University School of Medicine 333 Cedar Street, New Haven, CT 06510, USA

Received May 3, 1978. The cytoplasmic mRNA which codes for the herpes simplex virus specific thymidine kinase (TK) is polyadenylated and is 14.5s in size. This corresponds to an RNA of 1400 nucleotides. The TK polypeptide is about 42,000 daltons, which requires 1100 nucleotides. We conclude that the cytoplasmic mRNA is monocistronic. The reticulocyte lysate cell-free translation system synthesizes enzymatically active HSV-TK which can be assayed with high specificity and sensitivity by use of 125I-iododeoxycytidine as a substrate.


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