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Nucleic Acids Research, 1978, Vol. 5, No. 7 2373-2380
© 1978

Articles

A general method for the purification of restriction enzymes

Patricia J. Greene, Herbert L. Heyneker, Francisco Bolivar, Raymond L. Rodriguez, Mary C. Betlach, Alejandra A. Covarrubias, Keith Backman, David J. Russel, Robert Tait and Herbert W. Boyer

Department of Biochemistry and Biophysics, University of California San Francisco, San Francisco, CA 94143, USA

Received April 24, 1978. An abbreviated procedure has been developed for the purification of restriction endonucleases. This procedure uses chromatography on phosphocellulose and hydroxylapatite and results in enzymes of sufficient purity to permit their use in the sequencing, molecular cloning, and physical mapping of DNA.


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