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Nucleic Acids Research, 1978, Vol. 5, No. 8 2801-2808
© 1978

Articles

Genetic organization of the ribosomal transcription units of the yeast Saccharomyces carisbergensis

Jan H. Meyerink, Jan Retèl, Hendrik A. Raué, Rudi J. Planta, Arie van der Ende and Ernst F.J. van Bruggen

Biochem. Lab., Vrije Umversiteit De Boelelaan 1085, Amsterdam-Buitenveldert, Netherlands Biochem. Lab., Rijksuniversiteit Nijenborgh 16, 9747 AG Groningen, Netherlands

Received May 18, 1978. The genetic organization of the multiple ribosomal transcription units (RTUs) on the genome of the yeast Saccharomyces carlsbergensis was studied by electron microscopy of purified ribosomal DNA hybridized to 26S rRNA using the JR-loop technique (Thomas, M., White, R.L. and Davis, R.W. (1973) Proc.Natl.Acad.Sci.U.S. 73, 2294–2298). Plasmid pBR 322, the molecular weight of which is known, was used as a standard for converting contour length of double- stranded DNA into molecular weight.

The 140 yeast RTU5 were found to be arrayed in tandem repeats, each repeat containing at most 0.4 x 106 D (about 5% of the length of the RTU) of non-transcribed spacer DNA. The repeats, in turn, are arranged in a number of clusters separated by much longer str of non-ribosomal DNA.


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