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Nucleic Acids Research, 1978, Vol. 5, No. 8 2809-2824
© 1978

Articles

Chemical synthesis of a self-complementary octanucleotide, dG-G-T-T-A-A-C-C by a modified triester method

Kan L. Agarwal and Faina Riftina

Departments of Biochemistry and Biophysics and Theoretical Biology, The University of Chicago Chicago, IL 60637, USA

Received May 16, 1978. The synthesis of a self-complementary octanucleotide, d(G-G-T-T-A-A-C-C), using a modified triester approach is described. The protected dinucleotides, d(Me2O)TribG(ClC6H4) ibG, d(Me2O)TrT(ClC6H4)T, d(Me2O)TrT(ClC6H4)T, d(Me2O)TrbzA(ClC6H4)bzA and d(Me2O)TranC(ClC6H4)anC were synthesized by a one step triester procedure. After removal of the trityl group, the dinucleotides, dT(ClC6H4)T and danC (ClC6H4) were coupled to d(Me2O) and TribG(ClC6H4)ibG and d(Me2O) TrbzA(ClC6H4)bzA, respectively to yield the respective tetranucleotides. The tetranucleotide, d(Me2O)TrbzA(ClC6H4)bzA(ClC6H4)anC was detritylated and then coupled with d(Me2O)TribG(ClC6H4)ibG(ClC6H4)T(ClC6H4)T to give octanucleotide. The fully protected octanucleotide was deblocked by treatment with aqueous NH3 followed by acid and was characterized by nucleotide sequence analysis.


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