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Nucleic Acids Research, 1978, Vol. 5, No. 8 2999-3012
© 1978

Articles

Transcription of nucleosomes from human chromatin

Phyllis A. Shaw, Chintaman G. Sahasrabuddhe, Henry G. Hodo, III and Grady F. Saunders

Department of Biochemistry, The University of Texas System Cancer Center, M.D.Anderson Hospital and Tumor Institute, and The University of Texas Graduate School of Biomedical Sciences Houston, TX 77030, USA

Received May 12, 1978. Nucleosomes (chromatin subunits) prepared by micrococcal nuclease digestion of human nuclei are similar in histone content but substantially reduced in non-histone proteins as compared to undigested chromatin. Chromatin transcription experiments indicate that the DNA in the nucleosomes is accessible to DNA-dependent RNA polymerase in vitro The template capacities of chromatin and nucleosomes are 1.5 and 10%, respectively, relative to high molecular weight DNA, with intermediate values for oligonucleosomes. Three distinct sizes of transcripts, 150, 120 and 95 nucleotides in length, are obtained when nucleosomes are used as templates. However, when nucleosom DNA is used as a template, the predominant size of transcripts is 150 nucleotides. When oligonucleosomes are used as templates longer transcripts are obtained. This indicates that RNA polymerase can transcribe the DNA con tained in the nucleosomes.


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