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Nucleic Acids Research, 1978, Vol. 5, No. 9 3083-3100
© 1978

Articles

Characterization of two SV40 early mRNAs and evidence for a nuclear "prespliced" RNA species

Evelyne May, Michel Kress and Pierre May

Institut de Recherches Scientifiques sur le Cancer BP No 8, 94800 Villejuif, France

Received July 17, 1978. Using in vitro translation of sucrose-gradient fractionated cytoplasmic mRNA from SV40-infected cells, we have shown that a deletion in the region mapping between 0.54–0.59 reduced the size of mRNA for small-t but not the size of mRNA for large-T. Mutants with a deletion in this region were shown to produce in vivo either shortened small-t or no small-t, and normal large-T. Similarly, in vitro translation of poly(A)+cytoplasmic RNA from cells infected with these mutants gave the same results. On the other hand in vitro translation of poly(A)+nuclear RNA from the mutants which made no small-t produced a small-t derivative possibly synthesized from a prespliced RNA species. We have also shown that poly(A)+nuclear RNA from mutant d1 2122 produced two small-t related proteins: one of these (MW: 11K) probably represents the product of a "prespliced" RNA, the other (MW: 17K)which is also found in the cytoplasm represents the product of the mutant specific small-t mRNA.


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