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Nucleic Acids Research, 1978, Vol. 5, No. 9 3101-3112
© 1978

Articles

Characterization of an immunoglobulin cDNA clone containing the variable and constant regions for the MOPC 21 kappa light chain

Michael D. Strathearn1, Gary E. Strathearn1, Patricia Akopiantz1, Alvin Y. Liu1, Gary V. Paddock1 and Winston Salser1,2

1Department of Biology, University of California Los Angeles, CA 90024, USA 2Molecular Biology Institute, University of California Los Angeles, CA 90024, USA

Received July 14, 1978. Nucleotide sequence analysis and restriction endonuclease mapping have been used to characterize a cDNA copy of immunoglobulin MOPC 21 Kappa mRNA cloned in the bacterial plasmid pMB9. Three regions of the inserted cDNA of plasmid pL21–1 have been sequenced and match the known protein sequence at amino acid residues 1–24, 128–138 and 171–179. With these sequences to provide absolute correlations between the restriction map and the structural gene sequence it has been possible to exactly deduce the positions of all 11 of the insert restriction sites mapped within the structural gene. The pL21–1 insert contains the complete variable and constant regions as well as parts of the 3' untranslated and polypeptide leader coding sequences.


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