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Nucleic Acids Research, 1978, Vol. 5, No. 9 3347-3356
© 1978

Articles

Apurinic endonucleases from Saccharomyces cerevisiae

Paul R. Armel and Susan s. Wallace

Department of Microbiology, New York Medical College, Valhalla, NY 10595 USA

Received June 26, 1978. Three endonuclease activities have been partially purified from Saccharomyces cerevlsiae on the basis of their activity against x-irradiated closed-circular supercoiled bacteriophage PM2 DNA. These endonucleases also nick apurinic DNA and two out of the three activities incise DNA UV-irradiated with high doses. The endonuclease activities have also been distinguished on the basis of their magnesium requirement and sensitivity to EDTA.


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