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Nucleic Acids Research, 1980, Vol. 8, No. 10 2147-2164
© 1980

Articles

Nucleotide sequence of the operators of {lambda} ultravirulent mutants

A. Bailone* and F. Galibert

Laboratoire d'Hématologie Expérimentale Centre Hayem, Hôpital Saint-Louis, Paris 10è, France

Received March 31, 1980.

The nucleotide sequence of the operators of ultravirulent mutants of {lambda}, able to grow on host cells with elevated repressor levels, was determined. It appears that ultravirulence in {lambda}, requires multiple mutational events at the operator sequences. OL1, OL2 and OL3 operator sites are the target of mutational changes in ultravirulent phages indicating that these sites participate in vivo in repression of the PL promoter. No changes were found in the OR3 sequence, in contrast there is a mutation in OR2 and two mutations in OR1, in both {lambda}668 and {lambda}2668 phages. This mutated operator structure accounts for the constitutive expression of their PR promoter either in cells overproducing the {lambda} repressor or in cells overproducing the cro gene product.A model of the structure of the {lambda} operator site is proposed. The nucleotide sequence in each site can be divided into two functionally different subsets, one of which is recognized by the repressor while the other stabilizes the repressor-operator interaction.

*On leave of absence from Laboratoire d'Enzymologie, section de Radio-biologie Cellulaire, Centre National de la Recherche Scientifique, 91190 Gif-Gur-Yvette, France.


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