Skip Navigation

This Article
Right arrow Print PDF (921K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Welsh, J.D.
Right arrow Articles by Leibowitz, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Welsh, J.D.
Right arrow Articles by Leibowitz, M. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1980, Vol. 8, No. 11 2365-2376
© 1980

Articles

Transcription of killer virion double-stranded RNA in vitro

J.Douglas Welsh and Michael J. Leibowitz

Department of Microbiology, College of Medicine and Dentistry of New Jersey, Rutgers Medical School Piscataway, NJ 08854, USA

Received May 7, 1980. Intracellular virions of stationary phase ethanol-grown cells of a killer strain of Saccharomyces cerevisiae contain encapsulated M (1.1 x 106 dalton) and L (3.2 x 106 dalton) double-stranded RNA plasmids. These virions also contain RNA polymerase activity which catalyzes the synthesis of full-length, single-stranded, asymmetric transcripts (denoted m and 1) of the virion double-stranded RNAs. Product m is made by M-containing particles and shows complete sequence homology to M but not to L. Product 1 is made by L-containing particles and shows complete homology only to L. The products show no self-homology, indicating asymmetric transcription. Therefore, the polymerase appears to function in vitro as a double-stranded RNA transcriptase. The lack of sequence homology between M and L is confirmed.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.