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Nucleic Acids Research, 1980, Vol. 8, No. 11 2489-2498
© 1980

Articles

Characterization of Mg2+-induced conformational change in the 50S ribosomal subunit by differential hydrogen exchange

D. Bonnet, E. Begard*, M. Grunberg-Manago and G.Hui Bon Hoa*

Institut de Biologie Physico-Chimique 13, rue Pierre et Marie Curie, 75005, Paris, France *Groupe U 128, INSERM, B.P. 5051, 34033 Montpellier, France

Received April 22, 1980. The technique of differential hydrogen exchange allows detection of a conformational change in the 50S subunit of Escherichia coli ribosome when the magnesium concentration is lowered in a range where ribosomal activity is fully preserved. This change is characterized by a seventy-fold acceleration of about thirty labile hydrogens in the case of a Mg2+ jump from 10 mM to 2 mM. The small number of hydrogens involved can explain the difficulty in detecting this change by other methods.


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