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Nucleic Acids Research, 1980, Vol. 8, No. 13 2985-2998
© 1980

ENZYMOLOGY

Yeast viral RNA polymerase is a transcriptase

Jeremy Bruenn*, Libuse Bobek*, Victoria Brennan* and William Held+

*Division of Cell and Molecular Biology, State University of New York at Buffalo Buffalo, NY 14260, USA +Department of Viral Oncology, Roswell Park Memorial Institute Buffalo, NY 14263, USA

Received April 10, 1980. ScV-L is a simple double-stranded RNA virus of yeast, consisting of a 4.8 kilobase pair double-stranded RNA (L) encapsidated in isometric particles composed mainly of one polypeptide (ScV-Pl) of 88,000 daltons. L encodes ScV-Pl. There is a capsid-associated RNA polymerase that synthesizes in vitro predominantly single-stranded RNA. We show that this polymerase activity is a transcriptase, at least one product of which is the mRNA for ScV-Pl. The transcript, like its template, is uncapped.


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