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Nucleic Acids Research, 1980, Vol. 8, No. 13 3043-3054
© 1980

MOLECULAR BIOLOGY

The role of bacteriophage T7 gene 2 protein in DNA replication

Patrick Q. Mooney, R. North* and Ian J. Molineux+

Department of Microbiology, University of Texas Austin, TX 78712, USA

+ To whom correspondence should be addressed

Received May 7, 1980.

The in vivo function of the gene 2 protein of bacteriophage T7 has been examined. The gene 2 protein appears to modulate the activity of the gene 3 endonuclease in order to prevent the premature degradation of any newly-formed DNA concatemers. This modulation is not however a direct interaction between the two proteins. In single-burst experiments rifamycin can substitute for the gene 2 protein, allowing formation of fast-sedimenting replicative DNA intermediates and progeny phage production. This suggests that the sole function of the gene 2 protein is the inhibition of the host RNA polymerase and that the latter enzyme directs or promotes the endonucleolytic action of the gene 3 protein.

* current address: Burroughs Machines Limited, London, England


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