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Nucleic Acids Research, 1980, Vol. 8, No. 14 3087-3104
© 1980

MOLECULAR BIOLOGY

Post-transcriptional regulation of messenger abundance in rat liver and hepatoma

Howard Jacobs and G.D. Birnie

The Beatson Institute for Cancer Research Garscube Estate, Switchback Road, Bearsden, Glasgow, G61 1BD, UK

Received June 2, 1980. Saturation hybridisation with labelled single-copy INA shows that polysomal poly(A)+ RNA of rat liver and of a minimum-deviation rat hepatoma cell-line (HTC) have similar total complexities and that few sequences are specific to either cell-type. Hybridisation kinetics of polysomal cINAs with template and heterologous cell RNAs indicate that a proportion of liver messengers are at greatly reduced abundance in the hepatoma, but not the converse. Hybridisations using fractionated cINAs enriched for abundant polysomal sequences confirm these findings: on average, abundant liver mRNAe are about 100-fold rarer in hepatoma, whereas abundant hepatoma mRNAs are only 5-fold rarer in liver. This pattern is also implied by cell-free translation of polysomal poly(A)+ RNAs. The reactions of total and fractionated polysomal cINA probes with poly(A)+ muclear RNA indicate that there is much less disparity in abundance of these sequences at nuclear level, implying that the differences arise, at least in part, post- transcriptionally. We interpret the altered mRNA abundances in the hepatoma in terms both of its decreased functional specialisation and its ability to proliferate.


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