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Nucleic Acids Research, 1980, Vol. 8, No. 14 3125-3132
© 1980

ENZYMOLOGY

A new restriction endonuclease from the anaerobic bacterium, Desulfovibrio desulfuricans, Norway

Ronald A. Makula* and Richard B. Meagher**

*Department of Biochemistry, University of Georgia Athens, GA 30602, USA **Department of Genetics, University of Georgia Athens, GA 30602, USA

Received May 12, 1980. The purification and characterization of a new restriction endonu-clease, Dde 1, from a sulfate-reducing, anaerobic bacterium, Desulfovibrio desulfuricans, Norway, is reported. The enzyme recognizes the sequence


Formula

and cleaves at the position indicated by the arrows. The enzyme preparation obtained is suitable for restriction mapping and ligation.


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