Nucleic Acids Research, 1980, Vol. 8, No. 14 3133-3142
© 1980
ENZYMOLOGY |
Phy M: an RNase activity specific for U and A residues useful in RNA sequence analysis
Department of Microbiology and Molecular Genetics, Harvard Medical School Boston, MA 02115, USA
Received April 28, 1980.
Physarum polycephalum, an acellular slime mold, produces an endoribonuclease activity (Phy M) useful in direct RNA sequence determination. Under conditions previously described for direct enzymatic RNA sequencing (7M urea, 50° C, pH 5.0)1 Phy M cleaves almost exclusively and uniformly at Up
N and ApN. In the absence of urea UpN, ApN, and GpN are attacked in a sequence specific way identical to that found with RNase Phy I2 digests performed under the same reaction conditions.
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