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Nucleic Acids Research, 1980, Vol. 8, No. 15 3275-3285
© 1980

ENZYMOLOGY

A second site specific endonuclease from Thermus thermophilus 111, Tth111II

Takahisa Shinomiya, Mariko Kobayashi and Showbu Sato

Mitsubishi-Kasei Institute of Life Sciences 11 Minamiooya, Machida, Tokyo 194, Japan

Received June 20, 1980. A second site specific endonuclease with novel specificity has been purified from Thermus thermophilus strain 111 and named Tth111II. The enzyme is active at temperature up to 80°C and requires Mg2+ or Mn2+ for endonuclease activity. Tth111II cleaves {varphi}X174RFDNA into 11 fragments and lambda DNA into more than 25 fragments. From the 5'-terminal sequences of Tth111II fragments of {varphi}X174RFDNA determined by the two dimensional homochromatography and the survey on nucleotide sequence of {varphi}X174RFDNA, it was concluded that Tth111II recognizes the DNA sequence Formula and cleaves the sites as indicated by the arrows.


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