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Nucleic Acids Research, 1980, Vol. 8, No. 15 3459-3466
© 1980

ENZYMOLOGY

Sequence-specific interaction of a subunit of E. coli RNA polymerase with DNA

Walter Stender

Physiologisch-Chemisches Institut, Universität Würzburg Koelikerstrasse 2, D-8700 Würzburg, GFR

Received April 10, 1980. The interaction of {delta} subunit of E.coli RNA polymerase with DNA, either double or single-stranded, and with two inhibitors of RNA synthesis was investigated by using antibodies directed against the subunit. Free {delta} subunit was shown to interact with poly(dA), poly(dT), poly(dAC)-poly(dGT), T7 DNA and, to a lesser degree, with lambda DNA. When the {delta} subunit forms part of the holo enzyme, {delta} also interacts with poly(dG).poly(dC). Rifampicin and streptolydigin interact with {delta} in the holo enzyme and with free and core bound {delta} subunit, respectively. The results suggest that {delta} recognizes mainly AC-GT-sequences in double-stranded DNA. The findings are correlated with the base composition in RNA polymerase binding regions of promoters and suggest at least a general interaction between {delta} subunit and single-stranded DNA in open complexes.


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