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Nucleic Acids Research, 1980, Vol. 8, No. 16 3517-3534
© 1980

MOLECULAR BIOLOGY

Salt induced transitions of chromatin core particles studied by tyrosine fluorescence anisotropy

Louis J. Libertini and Enoch W. Small

Department of Biochemistry and Biophysics, Oregon State University Corvallis, OR 97331, USA

Received July 15, 1980. Chromatin core particles containing 146 base pairs of DNA have been found to undergo a single defined transition below 10 mM ionic strength as studied by both sedimentation velocity and tyrosine fluorescence anisotropy. A method is described for the preparation of such core particles from chicken erythrocytes with greater than 50% yield.


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