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Nucleic Acids Research, 1980, Vol. 8, No. 16 3553-3564
© 1980

MOLECULAR BIOLOGY

Brain tubutin and actin cDNA sequence: isolation of recombinant plasmids

I. Ginzburg*, A. de Baetselier*, M.D. Walker**, L. Behar*, H. Lehrach***, A.M. Frischauf*** and U.Z. Littauer*

*Department of Neurobiology, The Weizmann Institute of Science Rehovot, Israel **Department of Hormone Research, The Weizmann Institute of Science Rehovot, Israel ***European Molecular Biology Laboratory Heidelberg, GFR

Received June 30, 1980. Rat brain mRNA enriched for tubulin and actin sequences was used to prepare double stranded cDNA. A library of recombinant clones was constructed by inserting the dsDNA into the Pstl site of pBR322 plasmid and transformation of E.coli x1776 host. Clones bearing sequences coding for tubulin and actin were identified and characterized.


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