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Nucleic Acids Research, 1980, Vol. 8, No. 17 3743-3756
© 1980

MOLECULAR BIOLOGY

Nuctease digestion promotes structural rearrangements m H1-depleted chromatin

Wolfgang O. Weischet+ and K.E. Van Holde

Physiologisch-Chemisches Institut, Universitäts-Krakenhaus Eppendorf, 2000 Hamburg 20, GFR, and Department of Biochemistry and Biophysics, Oregon State University Corvallis, OR 97331, USA

+Reprint requests should be addressed to: Institut für Physiologische Chemie, Physikalische Biochemie und Zellbiologie der Universität München, Goethestr. 33, 8000 München 2, Federal Republic of Germany

Received July 4, 1980. Digestion of H1-depleted chromatin with micrococcal nuclease at an ionic strength of 0.35M gives rise to structural rearrangements indicating nucleosomal sliding. The ionic strength necessary to reveal this effect is significantly lower than that required in the absence of an accompanying digestion. As an explanation, a model is presented in which the progressing terminal degradation of oligomeric nucleosomes is made responsible for promoting structural rearrangements.


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