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Nucleic Acids Research, 1980, Vol. 8, No. 18 4185-4200
© 1980

MOLECULAR BIOLOGY

Affinity labeling of eukaryotic elongation factors using N{varepsilon}-bromoacetyl-Lys-tRNA

Arthur E. Johnson* and Lawrence I. Slobin{dagger}

*Department of Chemistry, University of Oklahoma Norman, OK 73019 {dagger}Department of Biochemistry, University of Mississippi Medical School Jackson, MS 39216, USA

Received August 11, 1980. eEF-T and eEF-Tu from rabbit reticulocyte and from Artemia were affinity labeled using N{varepsilon}-bromoacetyl-Lys-tRNA prepared with either yeast or E. coli tRNA. Only the eEF-Tu polypeptide was crosslinked when eEF-T was incubated with the reactive aminoacyl-tRNA analogue, which indicates that at least part of the aminoacyl-tRNA binding site is the same in both eEF-Tu and the multisubunit eEF-T. Complex formation (eEF-Tu·aa-tRNA·GTP) was required for crosslinking, since no covalent reaction with eEF-Tu occurred in the absence of GTP. The yield of crosslinked product was greatly reduced by adding either unmodified rabbit liver aminoacyl-tRNA or unmodified E. coli Lys-tRNA to the incubation to compete for the aminoacyl-tRNA binding site on eEF-T or eEF-Tu, indicating that the covalent reaction occurs while the N{varepsilon}-bromoacetyl-Lys-tRNA is bound in this site. The affinity labeling of a prokaryotic and two different eukaryotic elongation factors by the same reagent suggests that there may be conservation of structure in the region of the proteins which binds the aminoacyl end of the aminoacyl-tRNA.


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