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Nucleic Acids Research, 1980, Vol. 8, No. 19 4327-4336
© 1980

MOLECULAR BIOLOGY

Fusion of the promoter region of rRNA operon rrnB to lac Z gene

Gad Glaser*, Simi Kobi+ and Amos B. Oppenheim+

*Department of Cellular Biochemistry, Hebrew University - Hadassah Medical School Jerusalem, Israel +Department of Microbiological Chemistry, Hebrew University - Hadassah Medical School Jerusalem, Israel

Received August 20, 1980. A Lambda phage was constructed in which the structural gene for ß galactosidase is fused to a DNA segment carrying the ribosomal promoter rrnB of E. coli. In this hybrid operon ß galactosidase synthesis in vitro is repressed by ppGpp. Repression of ß galactosidase synthesis by cAMP is reported.


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