Nucleic Acids Research, 1980, Vol. 8, No. 2 319-335
© 1980
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Organization and expression of the mitochondrial genome of plants I. The genes for wheat mitochondrial ribosomal and transfer RNA: evidence for an unusual arrangement*
Department of Biochemistry, Dalhousie University, Halifax, Nova Scotia B3H 4H7, Canada
Received October 1, 1979.
We show here that mitochondrial-specific ribosomal and transfer RNAs of wheat (Triticium vulgare Vill. [Triticum aestivum L.] var. Thatcher) are encoded by the mitochondrial DNA (mtDNA). Individual wheat mitochondrial rRNA species (26S, 18S, 5S) each hybridized with several mtDNA fragments in a particular restriction digest (Eco RI, Xho I, or Sal I). In each case, the DNA fragments to which 18S and 5S rRNAs hybridized were the same, but different from those to which 26S rRNA hybridized. From these results, we conclude that the structural genes for wheat mitochondrial 18S and 5S rRNAs are closely linked, but are physically distant from the genes for wheat mitochondrial 26S rRNA. This arrangement of rRNA genes is clearly different from that in prokaryotes and chloroplasts, where 23S, 16S and 5S rRNA genes are closely linked, even though wheat mitochondrial 18S rRNA has previously been shown to be prokaryotic in nature. The mixed population of wheat mitochondrial 4S RNAs (tRNAs) hybridized with many large restriction fragments, indicating that the tRNA genes are broadly distributed throughout the mitochondrial genome, with some apparent clustering in regions containing 18S and 5S rRNA genes.
*An abstract of this work was presented at the N.A.T.O. Advanced Study Institute - F.E.B.S. Advanced Course on "Genome Expression and Organization in Higher Plants", Edinburgh, Scotland July 11-21, 1979.
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