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Nucleic Acids Research, 1980, Vol. 8, No. 2 361-375
© 1980

Article

Evidence implying DNA polymerase ß function in excision repair

J.A. Siedlecki, J. Szyszko*, I. Pietrzykowska* and B. Zmudzka

Institute of Oncology, Wawelska 15, 02-034 Warsaw, Poland *Institute of Biochemistry and Biophysics, Academy of Sciences, Rakowiecka 36, 02-532 Warsaw, Poland

Received December 14, 1979. Comparison was made of the ability of calf thymus DNA polymerases {alpha} and ß to replicate the following templates: native E. coli CR-34 DNA (T-DNA), calf thymus DNA activated by DNase I (act .DNA), BU-DHA (from E. coli CR-34 cells cultured on BUdR-containing medium) with damages resulting from incomplete excision repair, as well as thermally denatured act.DNA and BU-DNA (s.s.act.DNA and s.s.BU-DNA). 3H-TTP Incorparation during extensive replication of act.DNA was similar far both enzymes, being, as expected, 40 times higher than far T-DNA. Likewise, the differences in the yield of the s.s.act.DNA or s.S.BU-DNA replication between both enzymes were negligible. In contrast, damaged native DNA was 6–30 times more extensively replicated by DNA polymerase ß than {alpha}. We propose that this is due to the greater ability of DNA polymerase ß compared with {alpha} to replicate single-stranded gaps, the presence of which is more likely in damaged BU-DNA than in T-DNA and act.DNA.


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[Abstract] [Full Text] [PDF]


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